1SH2
Crystal Structure of Norwalk Virus Polymerase (Metal-free, Centered Orthorhombic)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.270, 115.240, 91.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.300 |
| R-factor | 0.23054 |
| Rwork | 0.227 |
| R-free | 0.29300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1khv |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.080 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.402 |
| Total number of observations | 215719 * | |
| Number of reflections | 27003 | 2054 * |
| <I/σ(I)> | 19.8 | 2.3 |
| Completeness [%] | 98.8 | 91.3 |
| Redundancy | 8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 8000, ammonium sulfate, Tris-Cl, glycerol, CHAPS, 2-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | polymerase | 7 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 24 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium sulfate | 100-200 (mM) | |
| 4 | 1 | reservoir | Tris-Cl | 50 (mM) | pH7.5 |
| 5 | 1 | reservoir | glycerol | 15 (%(w/v)) | |
| 6 | 1 | reservoir | CHAPS | 0.2 (%(w/v)) | |
| 7 | 1 | reservoir | 2-mercaptoethanol | 14 (mM) |
