1SGQ

GLY 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-01-09
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	45.480, 54.680, 45.550
Unit cell angles	90.00, 118.99, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.132 *
RMSD bond length	0.023 *
RMSD bond angle	2.500 *
Data reduction software	BIOMOL
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.043
Total number of observations	36307 *
Number of reflections	13125 *
Completeness [%]	84.9 *	36.5 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG6000	2 (%)
3	1	drop	sodium potassium phosphate	50 (mM)
4	1	reservoir	PEG6000	2 (%)
5	1	reservoir	sodium potassium phosphate	50 (mM)