1SGP

ALA 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Detector technology	DIFFRACTOMETER
Collection date	1993-11-28
Detector	WEISSENBERG
Spacegroup name	P 1 21 1
Unit cell lengths	45.380, 54.620, 45.470
Unit cell angles	90.00, 119.20, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.171
RMSD bond length	0.020
RMSD bond angle	2.400 *
Data reduction software	WEIS
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.400 *	1.400 *
Rmerge	0.073
Total number of observations	83250 *
Number of reflections	30783 *
Completeness [%]	80.3 *	56 *
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG6000	2 (%)
3	1	drop	sodium potassium phosphate	50 (mM)
4	1	reservoir	PEG6000	2 (%)
5	1	reservoir	sodium potassium phosphate	50 (mM)