1SGM
Crystal Structure of Hypothetical Protein YXAF
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9788, 0.9792, 0.930 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.356, 38.956, 95.705 |
Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
Resolution | 33.010 - 2.000 |
R-factor | 0.249 |
Rwork | 0.233 |
R-free | 0.29400 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | |
Number of reflections | 31966 | |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | BiS-Tris, MPD, PEG 1500, Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |