1SGH
Moesin FERM domain bound to EBP50 C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2000-05-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.943 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.600, 70.500, 62.800 |
| Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
| Resolution | 11.070 - 3.500 |
| Rwork | 0.338 |
| R-free | 0.40100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ef1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.750 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.070 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.101 | 0.471 |
| Number of reflections | 6801 | |
| <I/σ(I)> | 6.6 | 2 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Tris, PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
