1SFU
Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9203, 0.9159 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.203, 92.452, 48.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.237 |
| R-free | 0.27040 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.184 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 15772 | |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 1-1.1 M lithium sulfate, 2-4 mM magnesium chloride, 25 mM cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | lithium sulfate | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | lithium sulfate | ||
| 6 | 1 | 2 | magnesium chloride | ||
| 7 | 1 | 2 | H2O |
