1SFP

CRYSTAL STRUCTURE OF ACIDIC SEMINAL FLUID PROTEIN (ASFP) AT 1.9 A RESOLUTION: A BOVINE POLYPEPTIDE FROM THE SPERMADHESIN FAMILY

Experimental procedure

Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	41.550, 52.440, 48.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.173
Structure solution method	AB INITIO
RMSD bond length	0.006
RMSD bond angle	0.022
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-96
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.082 *
Total number of observations	44844 *
Number of reflections	8773
<I/σ(I)>	8.4
Completeness [%]	94.8	98
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	22 *	AMMONIUM SULFATE, PH 4.7, POLYETHYLENEGLYCOL 4000, 10% DIOXANE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)
3	1	reservoir	PEG2000	30 (%(w/v))
4	1	reservoir	ammonium sulfate	0.1 (M)