1SFK
Core (C) protein from West Nile Virus, subtype Kunjin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97956, 0.97976, 0.8856 |
| Spacegroup name | I 41 |
| Unit cell lengths | 85.655, 85.655, 214.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.25209 |
| Rwork | 0.249 |
| R-free | 0.31120 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.562 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.075 | 0.320 |
| Number of reflections | 12515 | |
| <I/σ(I)> | 21.2 | 3 |
| Completeness [%] | 99.4 | 95.3 |
| Redundancy | 5.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 288 | PEG3350, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
