1SES
CRYSTAL STRUCTURES AT 2.5 ANGSTROMS RESOLUTION OF SERYL-TRNA SYNTHETASE COMPLEXED WITH TWO DIFFERENT ANALOGUES OF SERYL-ADENYLATE
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.100, 125.200, 62.400 |
| Unit cell angles | 90.00, 108.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.300 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.066 * |
| Total number of observations | 170878 * |
| Number of reflections | 36920 * |
| Completeness [%] | 87.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 20 (%sat) | |
| 3 | 1 | drop | MES | 50 (mM) | |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | 1 (mM) | ||
| 7 | 1 | drop | MPD | 1 (%(v/v)) | |
| 8 | 1 | reservoir | ammonium sulfate | 30-35 (%sat) | |
| 9 | 1 | reservoir | MPD | 8 (%) |
