1SEP

MOUSE SEPIAPTERIN REDUCTASE COMPLEXED WITH NADP AND SEPIAPTERIN

Experimental procedure

Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-12
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	116.420, 116.420, 104.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.950
R-factor	0.176 *
Rwork	0.174
R-free	0.21600 *
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.729
Data reduction software	MOSFLM (V. 5.23)
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	1.950
High resolution limit [Å]	1.950	1.940
Rmerge	0.099
Total number of observations	304866 *
Number of reflections	31227
Completeness [%]	99.7	96.4
Redundancy	9.8	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.5 (mg/ml)
2	1	drop	MES	20 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	NADP	10 (mM)
5	1	reservoir	ammonium sulfate	1.5 (M)
6	1	reservoir	citrate	0.1 (M)