1SEL

CRYSTAL STRUCTURE OF SELENOSUBTILISIN AT 2.0-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	75.800, 65.400, 53.300
Unit cell angles	90.00, 107.30, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.173
Rwork	0.173
RMSD bond length	0.015
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	2.000 *	2.000 *
Rmerge		0.243 *
Number of reflections		5618 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	22.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	PEG8000	16-20 (%)
3	1	reservoir	imidazolemalate	0.2 (M)