1SEK
THE STRUCTURE OF ACTIVE SERPIN K FROM MANDUCA SEXTA AND A MODEL FOR SERPIN-PROTEASE COMPLEX FORMATION
Experimental procedure
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.960, 42.050, 76.050 |
Unit cell angles | 90.00, 117.60, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.26000 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Phasing software | X-PLOR (3.85) |
Refinement software | X-PLOR (3.85) |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.100 * | |
Rmerge | 0.070 * | |
Total number of observations | 43967 * | |
Number of reflections | 20609 * | |
Completeness [%] | 93.9 * | 66 * |
Redundancy | 2.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.8 * | 21 * | drop contains equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 28 (%) | |
3 | 1 | reservoir | 1.2 (M) | ||
4 | 1 | reservoir | EDTA | 1 (mM) | |
5 | 1 | reservoir | dithiothreitol | 5 (mM) | |
6 | 1 | reservoir | MES | 100 (mM) |