1SEB
COMPLEX OF THE HUMAN MHC CLASS II GLYCOPROTEIN HLA-DR1 AND THE BACTERIAL SUPERANTIGEN SEB
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991-11 |
| Detector | KODAK |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.000, 114.700, 149.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.700 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.32700 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.160 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 * |
| High resolution limit [Å] | 2.700 * |
| Rmerge | 0.057 |
| Number of reflections | 40627 |
| Completeness [%] | 86.0 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 25 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | reservoir | sodium acetate | 10 (mM) | |
| 4 | 1 | reservoir | ethylene glycol | 10 (%) | |
| 5 | 1 | reservoir | PEG4000 | 12-20 (%) |
