1SE8
Structure of single-stranded DNA-binding protein (SSB) from D. radiodurans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-10-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795, 0.9568 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.347, 63.476, 54.907 |
| Unit cell angles | 90.00, 106.27, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.800 |
| R-factor | 0.21789 |
| Rwork | 0.217 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.976 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 28011 | |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 200, PEG 4000, Sodium Acetate, Potassium Chloride, TCEP, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
