1SE4

STAPHYLOCOCCAL ENTEROTOXIN B COMPLEXED WITH LACTOSE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1993-07
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 21 21 21
Unit cell lengths	45.300, 71.000, 78.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.22
Rwork	0.220
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.013
RMSD bond angle	2.088
Data reduction software	FBSCALE ((LOCAL VERSION))
Data scaling software	FBSCALE ((LOCAL VERSION))
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.800
Number of reflections	17211
Completeness [%]	80.1
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.8		CRYSTALLIZED FROM 24% PEG 4000, 20 MM BORATE BUFFER AT PH 8.8 IN THE PRESENCE OF 100MM LACTOSE