1SDS

Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	F 2 2 2
Unit cell lengths	106.970, 141.300, 162.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.480 - 1.800
R-factor	0.207
Rwork	0.207
R-free	0.22500
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	56829
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 400, calcium chloride, HEPES, potassium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 400, calcium chloride, HEPES, potassium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K