1SDR
CRYSTAL STRUCTURE OF AN RNA DODECAMER CONTAINING THE ESCHERICHIA COLI SHINE-DALGARNO SEQUENCE
Experimental procedure
| Spacegroup name | P 1 |
| Unit cell lengths | 27.690, 30.900, 41.980 |
| Unit cell angles | 90.92, 103.63, 113.81 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.820 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | |
| High resolution limit [Å] | 2.600 | 2.600 * |
| Rmerge | 0.099 * | |
| Number of reflections | 3050 | |
| Completeness [%] | 83.6 | 81.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | 32 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 5 (mg/ml) | |
| 2 | 1 | drop | MPD | 5 (%(v/v)) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | sodium cacodylate | ||
| 5 | 1 | reservoir | MPD | 30 (%) | |
| 6 | 1 | reservoir | 400 (mM) | ||
| 7 | 1 | reservoir | sodium cacodylate | 40 (mM) |
