1SCU

THE CRYSTAL STRUCTURE OF SUCCINYL-COA SYNTHETASE FROM ESCHERICHIA COLI AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 2 2
Unit cell lengths	98.470, 98.470, 400.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.500
R-factor	0.216
Rwork	0.216
RMSD bond length	0.022
RMSD bond angle	3.250
Phasing software	X-PLOR
Refinement software	TNT

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.3 *	21 *	Wolodko, W.T., (1984) J. Biol. Chem., 259, 5316. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10 (mg/ml)
2	1	2	ammonium sulfate	1.9 (M)
3	1	2	potassium phosphate	0.1 (M)
4	1	2	dithiothreitol	1 (mM)
5	1	2	CoA	0.1 (mM)
6	1	2	EDTA	0.1 (mM)