1SCR

HIGH-RESOLUTION STRUCTURES OF SINGLE-METAL-SUBSTITUTED CONCANAVALIN A: THE CO,CA-PROTEIN AT 1.6 ANGSTROMS AND THE NI,CA-PROTEIN AT 2.0 ANGSTROMS

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	89.440, 87.210, 63.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.159
Rwork	0.159
RMSD bond length	0.013
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.040 *
Total number of observations	60304 *
Number of reflections	16435 *
Completeness [%]	97.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.5 *		Greer, J., (1970) J. Mol. Biol, 48, 365. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		0.1 (M)
2	1	1	Tris acetate	0.05 (M)