1SC5
Sigma-28(FliA)/FlgM complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.96486,0.97969,0.97989,0.96486 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.117, 118.117, 87.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.920 - 3.260 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.27100 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 11003 | |
| Completeness [%] | 96.5 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | PEG 800, sodium cacodylate, calcium acetate, sodium chloride, tris, isopropanol, 6-amino-caproic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
