1SBZ
Crystal Structure of dodecameric FMN-dependent Ubix-like Decarboxylase from Escherichia coli O157:H7
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9786, 0.9792, 0.9643 |
| Spacegroup name | H 3 |
| Unit cell lengths | 95.372, 95.372, 217.538 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.320 - 2.000 |
| R-factor | 0.18012 |
| Rwork | 0.176 |
| R-free | 0.21557 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 46319 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 85.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 15% PEG 4K, 0.2M Lithium sulphate, 0.1M Hepes (7.0), VAPOR DIFFUSION, HANGING DROP, temperature 22K |
