1SBR
The structure and function of B. subtilis YkoF gene product: the complex with thiamin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-16 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.820, 82.490, 86.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.20915 |
| Rwork | 0.206 |
| R-free | 0.26881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s99 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.669 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.306 |
| Number of reflections | 16658 | |
| <I/σ(I)> | 18.4 | 2.8 |
| Completeness [%] | 49.7 | |
| Redundancy | 4.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K, pH 6.50 |
