1SBQ

Crystal Structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae at 2.2 resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-13
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	42.315, 80.746, 132.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.200
R-factor	0.246
Rwork	0.239
R-free	0.26990
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.132
Number of reflections	23677
<I/σ(I)>	26.5
Completeness [%]	99.6	56.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	300	0.2 M ammonium sulfate, 0.1 M sodium acetate, 25% polyethylene glycol 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K