1SBK
X-RAY STRUCTURE OF YDII_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER29.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.642, 61.196, 93.008 |
| Unit cell angles | 90.00, 104.66, 90.00 |
Refinement procedure
| Resolution | 28.970 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1O0I |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.105 | 0.330 |
| Number of reflections | 34984 | |
| <I/σ(I)> | 13.3 | 4.1 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 303 | 1.75M Ammonium sulfate, 0.1M NaCl, 10mM DTT, 50 mM Imidazole pH 6.0, 3% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
