1SA0

TUBULIN-COLCHICINE: STATHMIN-LIKE DOMAIN COMPLEX

Experimental procedure

Experimental method	MIRAS
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-03
Detector	ADSC
Spacegroup name	P 65
Unit cell lengths	328.690, 328.691, 54.679
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.500 *
R-factor	0.23252
Rwork	0.232
R-free	0.25000 *
Structure solution method	MIRAS
RMSD bond length	0.014
RMSD bond angle	1.975
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.580
High resolution limit [Å]	3.500	3.500
Rmerge	0.062	0.639
Number of reflections	41617 *
Completeness [%]	96.4 *	82.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Gigant, B., (2000) Cell, 102, 809. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG