1S97
DPO4 with GT mismatch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-20 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.2834 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.433, 100.644, 110.426 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 29.640 - 2.400 |
| R-factor | 0.238 |
| Rwork | 0.218 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JX4:A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.010 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 | 0.397 |
| Number of reflections | 76535 | |
| <I/σ(I)> | 16.2 | 3 |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, ADA, ammonium acetate, calcium acetate, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ADA | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | calcium acetate | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | ADA | ||
| 7 | 1 | 2 | glycerol | ||
| 8 | 1 | 2 | H2O |
