1S94
Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0088 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.056, 73.056, 224.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.970 - 3.340 |
| R-factor | 0.31255 |
| Rwork | 0.309 |
| R-free | 0.37537 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ez3 chain A |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.610 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.074 | 0.375 |
| Number of reflections | 9304 | |
| <I/σ(I)> | 14.8 | 4.6 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 400, 0.2M Na-citrate, 0.1M Tris HCl, 20mM HEPES-KOH, 100mM KCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
