1S8L
Anion-free form of the D85S mutant of bacteriorhodopsin from crystals grown in the presence of halide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1272 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.725, 121.335, 74.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.970 - 2.300 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jv7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 10515 | |
| Completeness [%] | 96.9 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.6 | 298 | sodium acetate, PEG 4000, KCl, pH 4.6, lipidic cubic phase, temperature 298K |
