1S81
PORCINE TRYPSIN WITH NO INHIBITOR BOUND
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-06-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.423, 53.467, 46.605 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.700 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aks |
RMSD bond length | 0.008 |
RMSD bond angle | 1.930 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.119 * | 0.246 * |
Number of reflections | 21218 | |
<I/σ(I)> | 15.992 | 8.588 |
Completeness [%] | 98.0 | 100 |
Redundancy | 10.2 * | 9.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 4 * | 1.8M MgCl2, 50mM HEPES, 5mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | G3P | 100 (mM) | |
3 | 1 | drop | pH8.0 | ||
4 | 1 | reservoir | 1.6-1.8 (M) | ||
5 | 1 | reservoir | HEPES | 50 (mM) | or Tris |
6 | 1 | reservoir | 5 (mM) |