1S7Z

Structure of Ocr from Bacteriophage T7

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-01
Detector	MARRESEARCH
Wavelength(s)	1.2, 0.9777,0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	78.777, 37.665, 35.490
Unit cell angles	90.00, 98.42, 90.00

Refinement procedure

Resolution	38.900 - 1.830
R-factor	0.17349
Rwork	0.170
R-free	0.24100 *
Structure solution method	MAD
RMSD bond length	0.030 *
RMSD bond angle	2.200 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	1.880
High resolution limit [Å]	1.800 *	1.830
Rmerge	0.049	0.096
Total number of observations	71916 *
Number of reflections	8923
<I/σ(I)>	17.9	13.3
Completeness [%]	99.3 *	98.5
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	291	Sturrock, S.S., (2001) Acta Cryst., D57, 1652. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	32 (%)
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.2
3	1	reservoir		0.2 (M)
4	1	reservoir	glycerol	2.8 (%)
5	1	drop	protein	16 (mg/ml)