1S7O

Crystal structure of putative DNA binding protein SP_1288 from Streptococcus pygenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	127.608, 69.685, 55.247
Unit cell angles	90.00, 103.04, 90.00

Refinement procedure

Resolution	38.350 - 2.310
R-factor	0.2081
Rwork	0.207
R-free	0.23686
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.558
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.066	0.336
Number of reflections	40607
<I/σ(I)>	19	4
Completeness [%]	99.8	99.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.2	293	Citric Acid, Ammonium Nitrate, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K