1S7N
Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA free sulfhydryl)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-23 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.300, 82.300, 95.500 |
| Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7f |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 45918 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 93.7 | 86.6 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion under oil | 8 | 293 | pentaerythritiol propoxylate 426, triethanolamine, KCl, ammonium sulfate, acetylCoenzyme A, TCEP, pH 8.0, vapor diffusion under oil, temperature 293K |
