1S7D
Crystal structure of refined tetragonal crystal of YodA from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2002-07-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.009, 55.009, 153.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.450 - 2.170 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 13276 | |
| Completeness [%] | 99.9 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2M zinc acetate, 0.1M sodium carcodylate, 18% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
