1S76
T7 RNA polymerase alpha beta methylene ATP elongation complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 138.720, 143.320, 146.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.880 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.000 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.880 |
| Number of reflections | 33378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 21 * | T7 RNAP, DNA, RNA, alpha-beta methylene ATP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 50000 (nM) | pH7.4 |
| 2 | 1 | reservoir | PEG8000 | 6 (%) | |
| 3 | 1 | reservoir | 200000 (nM) | ||
| 4 | 1 | reservoir | magnesium acetate | 10000 (nM) | |
| 5 | 1 | reservoir | dithiothreitol | 10000 (nM) | |
| 6 | 1 | drop | Tris-HCl | 50000 (nM) | pH7.5 |
| 7 | 1 | drop | 20000 (nM) | ||
| 8 | 1 | drop | magnesium acetate | 10000 (nM) |
