1S6A
The X-ray structure of the cyanobacteria Synechocystis hemoglobin "cyanoglobin" with azide ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-06-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 |
Unit cell lengths | 83.350, 83.350, 65.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.400 - 1.690 |
R-factor | 0.19787 |
Rwork | 0.197 |
R-free | 0.21928 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | cyanide bound Synechocystis cyanoglobin PDb entry 1S69 |
Data reduction software | CRYSTAL (VIEW) |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.680 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.048 | 0.257 |
Number of reflections | 34919 | |
<I/σ(I)> | 9.9 | 2.4 |
Completeness [%] | 70.5 | 9.9 |
Redundancy | 1.73 | 1.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 1.5M sodium citrate, 20% sucrose, 50mM sodium azide, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |