1S67

Crystal structure of heme domain of direct oxygen sensor from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	47.575, 67.690, 82.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.223
Rwork	0.197
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.560
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.500
Rmerge	0.055
Number of reflections	34562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	PEG, Na Tartarate, Na acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K