1S5Y
The crystal structure of Trematomus bernacchii hemoglobin oxidized by ferricyanide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-10 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.993, 62.980, 63.502 |
| Unit cell angles | 77.06, 69.79, 84.17 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.203 |
| Rwork | 0.199 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.106 | 0.398 |
| Number of reflections | 23515 | |
| Completeness [%] | 87.2 | 69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.6 | 293 | MPEG 5000, Tris, pH 7.6, LIQUID DIFFUSION, temperature 293K |
