1S5X
The crystal structure of Trematomus bernacchii hemoglobin oxidized by air
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-02 |
| Detector | MAC Science DIP-2030B |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.516, 65.095, 55.750 |
| Unit cell angles | 90.00, 113.48, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.195 |
| Rwork | 0.190 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.346 | |
| Number of reflections | 14020 | |
| Completeness [%] | 99.5 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.6 | 293 | MPEG 5000, Tris, pH 7.6, LIQUID DIFFUSION, temperature 293K |
