Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

1S5J

Insight in DNA Replication: The crystal structure of DNA Polymerase B1 from the archaeon Sulfolobus solfataricus

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-2
Synchrotron siteESRF
BeamlineID14-2
Temperature [K]100
Detector technologyCCD
Collection date2000-10-04
DetectorADSC QUANTUM 4
Wavelength(s)0.9326
Spacegroup nameC 1 2 1
Unit cell lengths187.470, 68.800, 125.850
Unit cell angles90.00, 107.94, 90.00
Refinement procedure
Resolution30.000 - 2.400
R-factor0.23443
Rwork0.232
R-free0.27222
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.012
RMSD bond angle1.268
Data reduction softwareDENZO
Data scaling softwareCCP4 ((SCALA))
Phasing softwareAMoRE
Refinement softwareREFMAC (5.1.24)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.460
High resolution limit [Å]2.4002.400
Rmerge0.114
Number of reflections59688
<I/σ(I)>4.1
Completeness [%]100.086.7
Redundancy5.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8294ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K

229380

PDB entries from 2024-12-25

PDB statisticsPDBj update infoContact PDBjnumon