1S5J
Insight in DNA Replication: The crystal structure of DNA Polymerase B1 from the archaeon Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9326 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.470, 68.800, 125.850 |
| Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.23443 |
| Rwork | 0.232 |
| R-free | 0.27222 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.268 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.114 | |
| Number of reflections | 59688 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 100.0 | 86.7 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | ammonium sulphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
