1S58
The structure of B19 parvovirus capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.107 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 351.393, 351.393, 351.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.313 |
| Rwork | 0.313 |
| R-free | 0.31600 |
| Structure solution method | molecular replacement + NCS averaging |
| Starting model (for MR) | cryo-electron microscopy reconstruction |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | ENVELOPE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.098 | 0.436 |
| Number of reflections | 123514 | |
| <I/σ(I)> | 7.2 | 1.38 |
| Completeness [%] | 68.0 | 52.6 |
| Redundancy | 1.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 20% MPD, 0.1M Na Cacodylate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
