1S4V
The 2.0 A crystal structure of the KDEL-tailed cysteine endopeptidase functioning in programmed cell death of Ricinus communis endosperm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.448, 68.162, 163.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.300 * - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cqd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.469 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.400 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 * | 0.137 * |
| Total number of observations | 179209 * | |
| Number of reflections | 28159 | |
| Completeness [%] | 98.5 | 89.3 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 * | 291 | PEG 4000, Li2SO4, pH 5.04, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | MES/NaOH | 3 (mM) | pH5.5 |
| 3 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 31 (%(w/v)) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.04 |
| 6 | 1 | reservoir | 0.2 (M) |
