1S4Q
Crystal Structure of Guanylate Kinase from Mycobacterium tuberculosis (Rv1389)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 112.126, 112.126, 112.126 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.060 - 2.160 |
| R-factor | 0.17988 |
| Rwork | 0.177 |
| R-free | 0.23071 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.890 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Number of reflections | 12112 | |
| <I/σ(I)> | 36.6 | 4.94 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.91 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | sodium formate, glycerol, Tris, sodium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
