1S2J
Crystal structure of the Drosophila pattern-recognition receptor PGRP-SA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-06-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.91963 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.733, 49.431, 69.830 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.200 |
| R-factor | 0.19572 |
| Rwork | 0.193 |
| R-free | 0.24179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1OHT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 | 0.140 |
| Number of reflections | 20080 | |
| <I/σ(I)> | 18.6 | 387.9 |
| Completeness [%] | 99.7 | 95 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 294 | sodium potassium phosphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
