1S2B

Structure of SCP-B the first member of the Eqolisin family of Peptidases to have its structure determined

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	108.601, 108.601, 114.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.230
R-free	0.24600
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Rmerge	0.058
Number of reflections	18539
<I/σ(I)>	8.1
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	42% ammonium sulphate, 0.1M sodium acetate, 10% v/v ethelene glycol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K