1S29
La autoantigen N-terminal domain
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-08-15 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.926, 53.912, 53.833 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.600 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.169 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.590 * |
| Rmerge | 0.032 | 0.060 |
| Number of reflections | 24672 * | 2382 * |
| <I/σ(I)> | 41 | 21 |
| Completeness [%] | 99.5 | 95.7 |
| Redundancy | 7.0 * | 4.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG3500, GLYCEROL, HEPES, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 2 | 1 | reservoir | glycerol | 5 (%(v/v)) | |
| 3 | 1 | reservoir | PEG3500 | 32-36 (%(w/v)) | |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | protein | 10-20 (mg/ml) |
