1S1Z
Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 to 500 nanoseconds
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.917, 66.917, 41.002 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.600 |
| R-factor | 0.05 |
| Rwork | 0.051 |
| R-free | 0.06000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | LaueView |
| Data scaling software | LaueView |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 13701 | |
| Completeness [%] | 98.1 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium sulfate, sodium phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium sulfate, sodium phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium sulfate, sodium phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium sulfate, sodium phosphate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
