1S1Y

Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	288
Detector technology	CCD
Collection date	2001-03-23
Detector	ADSC QUANTUM 4
Spacegroup name	P 63
Unit cell lengths	66.917, 66.917, 41.002
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 - 1.600
R-factor	0.0655
Rwork	0.064
R-free	0.07020
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	LaueView
Data scaling software	LaueView
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.670
High resolution limit [Å]	1.600	1.600
Number of reflections	13371
Completeness [%]	95.8	83.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	ammonium sulfate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	ammonium sulfate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	ammonium sulfate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	ammonium sulfate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K