1S0P

Structure of the N-Terminal Domain of the Adenylyl Cyclase-Associated Protein (CAP) from Dictyostelium discoideum.

Replaces:  1PN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-04-30
Detector	RIGAKU
Wavelength(s)	0.9760, 0.9176, 1.105
Spacegroup name	P 1
Unit cell lengths	37.520, 42.130, 53.740
Unit cell angles	97.42, 105.09, 97.15

Refinement procedure

Resolution	20.000 * - 1.400
R-factor	0.26
Rwork	0.180
R-free	0.21000
Structure solution method	SIRAS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.800
High resolution limit [Å]	1.400	1.400
Rmerge	0.032 *	0.128 *
Total number of observations	3602228 *
Number of reflections	58737 *
Completeness [%]	93.7 *	92.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.1	277	30% PEG8000, 0.2 M MgCl2, 10 mM -mercaptoethanol, and 0.1 M MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	30 (%)
2	1	reservoir		0.2 (M)
3	1	reservoir	beta-mercaptoethanol	10 (mM)
4	1	reservoir	MES	0.1 (M)	pH6.1