1S0N
Snapshots of replication through an abasic lesion: structural basis for base substitution and frameshift
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-09-10 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 99.112, 102.660, 53.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jx4 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG3350, CA(AC)2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG3350 | ||
2 | 1 | 1 | CA(AC)2 | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | PEG3350 | ||
5 | 1 | 2 | CA(AC)2 | ||
6 | 1 | 2 | H2O |