1S03
The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Wavelength(s) | 1.0049 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.220, 61.220, 332.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.23734 |
| Rwork | 0.237 |
| R-free | 0.27313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j5e |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 20950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 296 | PEG 8000, zinc acetate, sodium acetate buffer, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 296 | PEG 8000, zinc acetate, sodium acetate buffer, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 296 | PEG 8000, zinc acetate, sodium acetate buffer, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | zinc acetate | ||
| 3 | 1 | 1 | sodium acetate buffer | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 800 | ||
| 6 | 1 | 2 | zinc acetate | ||
| 7 | 1 | 2 | sodium acetate buffer | ||
| 8 | 1 | 2 | H2O |
